Genome
Sequencing
Transcriptome Sequencing
Epigenome Sequencing
Library Sequencing
Library Sequencing
With the advanced sequencing platforms of Illumina and PacBio, we provide sequencing-only services for pre-prepared libraries with various read lengths and capacities, tailored to different budgets, project sizes, and turnaround times.
Lifescience accepts customer-prepared libraries for sequencing using multiple platforms that offer varying read lengths, throughput, and turnaround options. We directly sequence customer-supplied libraries and generate high-quality sequencing data that forms a solid foundation for subsequent bioinformatics analyses. Sequencing is supported on the Illumina NovaSeq series with 10B, 25B, and SP flow cell options, including partial and full lane sequencing, as well as complete PacBio SMRT cell sequencing. Our professional and efficient operations team ensures timely project completion and high data quality.
We continuously strive to provide fast and convenient sequencing services to our customers. By offering scientific support, stringent quality control management, extensive project experience, and exceptional service, we ensure that every step of your research project is executed flawlessly. With our world-leading capacity including Illumina and PacBio platforms, we offer highly competitive pricing for sequencing services on pre-prepared libraries.
Benefits of Pre-prepared Library Sequencing
- Advanced NGS technologies: Lifescience utilizes cutting-edge platforms such as Illumina NovaSeq X Plus and PacBio Revio.
- Diverse sequencing strategies: Supports various options including NovaSeq X PE150, PE250, PE50, SE50, and PacBio SMRT HiFi.
- Extensive experience: Thousands of projects with data published in prestigious journals like Cell, Nature, and Science.
- Fast turnaround times: Laboratories in Germany and the UK reduce shipping times and accelerate processing.
- Competitive pricing: High-quality sequencing services for pre-prepared libraries offered at affordable costs.
- Scientific support: Expert assistance and rigorous quality control management provided at every step.
| Platform | Data Amount | Volume Requirement | Library Concentration |
|---|---|---|---|
| NovaSeq X Plus PE150 | <30 GB | ≥15 µL | ≥ 2 ng/µL, quantified using Qubit® 2.0 (Life Technologies) ≥ 2 nM, quantified by qPCR |
| 30 GB ≤ X < 100 GB | ≥30 µL | ||
| 100 GB ≤ X < 375 GB | ≥70 µL | ||
| 375 GB ≤ X < 1 TB | ≥130 µL | ||
| 10B flow cell sequencing | ≥70 µL (additional 70 µL per extra flow cell) | ||
| 25B flow cell sequencing | ≥130 µL (additional 130 µL per extra flow cell) | ||
| NovaSeq seriesSE50/PE50/PE250 | <20 M reads | ≥15 µL | ≥ 2 ng/µL, quantified with Qubit® 2.0 (Life Technologies) ≥ 2 nM, quantified with qPCR |
| 20 M reads ≤ X < 50 M reads | ≥25 µL | ||
| 50 M reads ≤ X < 150 M reads | ≥50 µL | ||
| Flow cell sequencing | ≥70 µL (additional 70 µL per extra flow cell) |
Notes: Highly concentrated samples should be diluted prior to submission. Pre-prepared libraries must be colorless.