Genome
Sequencing
Transcriptome Sequencing
Epigenome Sequencing
Library Sequencing
Epigenome Analysis
Epigenome analysis examines mechanisms that regulate gene expression without altering the underlying DNA sequence, providing deep insights into cellular functions and disease processes. Epigenetic modifications such as DNA methylation and histone modifications play critical roles in gene regulation and are involved in a wide range of biological processes from developmental biology to cancer.
Lifescience, offers comprehensive epigenomic sequencing solutions optimized for specific research needs. Our service range includes:
- Whole Genome Bisulfite Sequencing (WGBS): Provides C-level DNA methylation profiling.
- Enzymatic Methyl-seq (EM-seq): A sensitive, low-damage alternative to traditional bisulfite methods.
- Reduced Representation Bisulfite Sequencing (RRBS): Targeted sequencing for cost-effective methylation analysis.
- Long-Read Based Methylation Detection: Comprehensive methylation analysis using PacBio SMRT and Oxford Nanopore platforms.
- ChIP-seq: Chromatin immunoprecipitation sequencing to analyze DNA-protein interactions and binding sites.
- RIP-seq: RNA immunoprecipitation sequencing to reveal RNA-protein interactions.
- ATAC-seq: Identifies open chromatin regions to analyze chromatin accessibility at bulk and single-cell levels.
Decoding epigenetic mechanisms is critical for agricultural research, disease mechanism elucidation, drug discovery, and personalized treatment strategies. LifeScience provides expert support throughout every step of the analysis process so that you can focus solely on utilizing the results.
Epigenome Sequencing
- Whole Genome Methylation Sequencing
- lncRNA Sequencing
- Reduced Representation Bisulfite Sequencing (RRBS)
- Chromatin Immunoprecipitation
Sequencing (ChIP-seq) - RNA Immunoprecipitation
Sequencing (RIP-seq)
Whole genome methylation sequencing enables detailed analysis of epigenetic modifications such as 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), which have critical effects on gene regulation, cellular development, and genomic stability. LifeScience offers comprehensive and reliable genome-wide methylation detection solutions tailored to your specific research goals. This service is a powerful tool to understand the mechanisms of epigenetic regulation and to gain in-depth insights in biomedical, agricultural, or developmental studies.
| Service | Sample Type | Quantity (Qubit) | Volume | Concentration | Purity |
|---|---|---|---|---|---|
| Mikrobial WGS | Genomic DNA | ≥ 200 ng | ≥ 20 µL | ≥ 10 ng/µL | – |
| Shotgun-based Metagenomics | Total DNA | ≥ 200 ng | ≥ 20 µL | ≥ 20 ng/µL | OD260/280 = 1.8–2.0 No degradation, no contamination |
| PCR-free WGS/Shotgun Metagenomics | Genomic DNA / Total DNA | ≥ 1.2 µg | ≥ 20 µL | ≥ 20 ng/µL | – |
| Amplicon-based Metagenomics (Illumina) | Total DNA | ≥ 200 ng | ≥ 20 µL | ≥ 10 ng/µL | – |
| PacBio Revio HiFi Sequencing | HMW Genomic DNA | ≥ 5 µg | ≥ 50 µL | ≥ 70 ng/µL | OD260/280 = 1.7–2.2 OD260/230 = 1.3–2.6 NC/QC = 1.00–2.20 Fragments≥ 20K |
| PacBio Sequel IIe CLR Sequencing | HMW Genomic DNA | ≥ 2 µg | ≥ 50 µL | ≥ 70 ng/µL | OD260/280 = 1.7–2.2 OD260/230 = 1.3–2.6 NC/QC = 0.95–3.00 Fragments≥ 20K |
| PacBio Kinnex 16S Amplicon | Isolated DNA | ≥ 300 ng | ≥ 30 µL | ≥ 10 ng/µL | OD260/280 = 1.8–2.0 No degradation, no contamination |
| PacBio MAS-seq 18S/ITS | Isolated DNA | ≥ 300 ng | ≥ 30 µL | ≥ 10 ng/µL | – |
| Oxford Nanopore PromethION | HMW Genomic DNA | ≥ 6 µg | ≥ 50 µL | ≥ 60 ng/µL | OD260/280 = 1.7–2.2 OD260/230 = 1.3–2.6 NC/QC = 0.95–3.00 Fragments≥ 20K |
Long non-coding RNAs (lncRNAs) are a type of non-coding RNA longer than 200 nucleotides (nt) that constitute a significant portion of the eukaryotic transcriptome. This group includes various subtypes such as intergenic lncRNAs (lincRNAs), intronic, antisense, sense, and bidirectional lncRNAs.
LncRNAs have multifaceted roles in cellular functions through the regulation of gene transcription, post-transcriptional modifications, and epigenetic mechanisms. They play critical roles particularly in gene expression control and cell differentiation processes.
LncRNA sequencing (lncRNA-seq) is a powerful Next-Generation Sequencing (NGS) method used to investigate the functional roles of lncRNAs in many human diseases, especially cancer and neurological disorders.
| Service | Sample Type | Quantity (Qubit) | Volume | Concentration | RIN (Agilent 2100) | Purity |
|---|---|---|---|---|---|---|
| Eukaryotic Strand-specific lncRNA (rRNA removal) | Total RNA (animal) | ≥ 500 ng | ≥ 10 µL | ≥ 50 ng/µL | ≥ 5.5, with flat baseline | – |
| Total RNA (plant & fungi) | ≥ 500 ng | ≥ 10 µL | ≥ 50 ng/µL | ≥ 5.5, with flat baseline | – | |
| Total RNA (blood) | ≥ 500 ng | ≥ 10 µL | ≥ 50 ng/µL | ≥ 5.5, with flat baseline | – | |
| Exosome RNA (human & mouse) | ≥ 5 ng | ≥ 10 µL | – | Fragments between 25–200 nt, FU* > 10 | – | – |
| Eukaryotic sRNA (18–40 bp insert) | Total RNA (animal) | ≥ 2 µg | ≥ 20 µL | ≥ 50 ng/µL | ≥ 7.5, with flat baseline | OD260/280 ≥ 2.0; OD260/230 ≥ 2.0; no degradation, no contamination |
| Total RNA (plant & fungi) | ≥ 2 µg | ≥ 20 µL | ≥ 50 ng/µL | ≥ 7.0, with flat baseline | ||
| Exosome RNA | ≥ 10 µg | ≥ 10 µL | – | Fragments between 25–200 nt, FU* > 10 | ||
| Eukaryotic circRNA (rRNA and linear RNA removal) |
Total RNA (animal) | ≥ 2 µg | ≥ 20 µL | ≥ 50 ng/µL | ≥ 7.0, with flat baseline | |
| Total RNA (plant & fungi) | ≥ 2 µg | ≥ 20 µL | ≥ 50 ng/µL | ≥ 6.5, with flat baseline | ||
| Eukaryotic lncRNA & sRNA | Total RNA | ≥ 2.5 µg | ≥ 30 µL | ≥ 50 ng/µL | ≥ 7.5 with flat baseline (hayvan) ≥ 7.0 with flat baseline (bitki & mantar) |
– |
| Eukaryotic lncRNA, sRNA & circRNA | Total RNA | ≥ 4.5 µg | ≥ 50 µL | ≥ 50 ng/µL | – | – |
*Fluorescent units
Reduced Representation Bisulfite Sequencing (RRBS) is an effective and targeted approach for DNA methylation analysis, focusing particularly on CpG-rich regions such as CpG islands. The RRBS method implemented by Novogene aims to obtain high-resolution data specifically from these regions where methylation is most abundant in mammalian genomes.
In this method, first, the methylation-insensitive restriction enzyme MspI is used to identify and isolate CpG-rich regions. The selected DNA fragments are then treated with bisulfite. During this process, unmethylated cytosines are converted to uracil, while methylated cytosines remain unchanged. By comparing the resulting sequences to the reference genome, the methylation status of each cytosine can be precisely determined.
RRBS provides highly accurate methylation data from limited amounts of DNA and serves as a reliable analytical tool in many research areas, including epigenetic regulation, gene expression control, and understanding disease mechanisms.
| Service | Sample type | Amount (Qubit) | Volume | Concentration | Purity |
|---|---|---|---|---|---|
| ChIP-seq | Enriched DNA | ≥ 5 ng | ≥ 10 µL | – | Main peak within 100 bp and 500 bp |
| RIP-seq | Enriched DNA | ≥ 20 ng | ≥ 10 µL | ≥ 2 ng/µL | Fragments longer than 80 nt |
| Whole Genome Bisulfite Sequencing (WGBS) | Genomic DNA | ≥ 100 ng | ≥ 20 µL | ≥ 5 ng/µL | 0 > OD260/230 > 3; no degradation, no contamination |
| Reduced Representation Bisulfite Sequencing (RRBS) | Genomic DNA | ≥ 800 ng | ≥ 20 µL | ≥ 40 ng/µL | – |
| Enzymatic Methylation Sequencing (EM-Seq) | Genomic/FFPE* DNA | ≥ 100 ng | ≥ 20 µL | ≥ 5 ng/µL | 0 > OD260/230 > 3; No EDTA in solvent |
| cfDNA/ctDNA** | ≥ 20 ng | – | ≥ 3 ng/µL | No EDTA in solvent |
* Formalin-Fixed, Paraffin-Embedded
** Cell-free DNA, circulating-tumor DNA
ChIP-seq (Chromatin Immunoprecipitation Sequencing) is a powerful epigenetic analysis technique used to map the binding sites of DNA-interacting proteins across the genome. This method enables high-resolution identification of the distribution of transcription factors, histone modifications, and other chromatin-associated proteins on target DNA regions.
ChIP-seq combines the specificity of chromatin immunoprecipitation (ChIP) to isolate specific protein-DNA complexes with next-generation sequencing (NGS) technology to sequence the DNA fragments obtained from these complexes. This approach provides deeper insights into intracellular gene regulation mechanisms and reveals how gene expression is controlled at the epigenetic level.
ChIP-seq is widely used in studies focused on transcriptional regulation, cell differentiation, disease mechanisms, and epigenetic inheritance.
| Service | Sample Type | Amount (Qubit) | Volume | Concentration | Purity |
|---|---|---|---|---|---|
| ChIP-seq | Enriched DNA | ≥ 5 ng | ≥ 10 µL | – | Main peak within 100 bp and 500 bp |
| RIP-seq | Enriched DNA | ≥ 20 ng | ≥ 10 µL | ≥ 2 ng/µL | Fragments longer than 80 nt |
ChIP-seq Highlights
- Understand how transcription factors regulate genes by profiling genome-wide binding sites
- Outline histone modification patterns associated with experimental treatment or sample conditions
- Investigate the relationships between epigenetic profiles and transcriptional regulation through joint analysis
- Explore correlations between ChIP-seq and gene expression through associated analysis
RIP-seq Highlights
- Verify the interactions between RNAs and target proteins
- Analyze the interaction network between RNA binding proteins and ncRNAs, such as lncRNA and miRNA
- Identify genome-wide networks of RNA-RBP interactions
RIP-seq (RNA Immunoprecipitation Sequencing) is a powerful next-generation sequencing technique used to analyze RNA molecules interacting with specific RNA-binding proteins (RBPs). This method enables genome-wide mapping of protein-RNA interactions. It provides researchers with the ability to investigate in detail how RBPs play roles in fundamental biological processes such as gene expression, RNA splicing, stability, intracellular localization, and translation. Thus, RIP-seq serves as an important tool for identifying gene regulation mechanisms and RBP targets related to diseases.
| Service | Sample Type | Amount (Qubit) | Volume | Concentration | Purity |
|---|---|---|---|---|---|
| ChIP-seq | Enriched DNA | ≥ 5 ng | ≥ 10 µL | – | Main peak within 100 bp and 500 bp |
| RIP-seq | Enriched DNA | ≥ 20 ng | ≥ 10 µL | ≥ 2 ng/µL | Fragments longer than 80 nt |
ChIP-seq highlights
- Understand how transcription factors regulate genes by profiling a genome-wide binding sites
- Outline histone modification patterns associated with experimental treatment or sample conditions
- Investigate the relationships between epigenetic profiles and transcriptional regulation through joint analysis
- Associated analysis is provided to explore correlations between ChIP-seq and gene expression
RIP-seq highlights
- Verify the interactions between RNAs and target proteins
- Analyse the interaction network between RNA binding proteins and ncRNAs, such as lncRNA and miRNA
- Identify genome-wide networks of RNA-RBP interactions